CHO DG44 Cells (cGMP banked) and Media Kit
Description
The CHO DG44 Cells (cGMP banked) and Media Kit is optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. The kit provides:GIBCO CD DG44 Medium—a chemically defined, protein-free medium specifically designed to enable optimal growth and expression of DG44 cells in suspension culture
CHO DG44 cells (cGMP banked) —Parental CHO DG44 cells have been produced, banked and tested to meet cGMP quality standards. They are pre-adapted to CD DG44 Medium and selected for superior cell growth and transfection efficiencies.
In addition, the CHO DG44 Cells (cGMP banked) and Media Kit includes L-glutamine and Pluronic F-68 for optimal growth and protein expression of DG44 cells in a protein-free, chemically defined environment.
Specifications
| Species: | Hamster |
| Cell Line: | CHO DG44 |
| Classification: | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Culture Type: | Suspension Cell Culture |
| Endotoxin Level: | Low |
| Glutamine: | No Glutamine |
| Included Antibiotics: | No antibiotics |
| Media Adaptation: | CD DG44 Medium |
| Media Recommendation: | CD DG44 Medium |
| Number of Cells: | 1 x 107 |
| Parental Cells: | DG44 |
| Phenol Red Indicator: | No Phenol Red |
| Product Line: | Gibco |
| Product Size: | 1 kit |
| Sodium Bicarbonate Buffer: | Sodium Bicarbonate |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Stably Integrated Gene or Element: | dhfr- |
| Supplements: | Hypoxanthine, Thymidine |
Contents & storage
CHO DG44 Cells (cGMP banked) and Media Kit (SKU A1100001) contains:
- CD DG44 Medium 1000 mL- 12610-010, store in the dark at 2-8°C
- CHO DG44 Cells (cGMP banked) 1 vial (> 1x10^7 cells) - A1097101 (see limited label license), store in liquid nitrogen
- PLURONIC F-68, 10% (100X) 100 mL - 24040-032, store at room temperature
- L-glutamine-200mM (100X) 100 mL - 25030-081 or 25030-024 (CE marked version for Europe only), store at -20°C
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