Guanidine-HCl
Description
Thermo Scientific Pierce Guanidine-HCl can be easily dissolved and pH-adjusted to any concentration.Features of Guanidine-HCl:
• No wasting valuable research time weighing and dissolving guanidine crystals
• Free from UV absorbing materials in the range of 225-300nm
• Free from heavy metal contaminants
• Free from degradation products such as ammonia
• Excellent stability
Applications of Guanidine Hydrochloride:
• Solubilizing proteins from inclusion bodies
• Increasing solubility of hydrophobic peptides and proteins
• Denaturing proteins
Chaotropes, substances that disrupt the structure of water interactions, help to solubilize hydrophobic proteins and peptides for a variety of biological purposes. For example, hydrophobic peptides and proteins may be dissolved in coupling buffer containing either 6M guanidine-HCl or 4M urea prior to immobilization on AminoLink Plus Support to create an affinity column. However, because the degradation products of guanidine and urea include ammonia, which contains a primary amine that will compete for reaction to the support, urea and guanidine solutions must be prepared with high quality reagents immediately before use.
Guanidine is a general protein denaturant, unfolding proteins and altering their three-dimensional structure. Consequently, some proteins will be irreversibly altered upon interaction with guanidine solutions and may lose their binding function. Before any large-scale use of guanidine, it is best to test a small sample and determine whether the denaturing effects will adversely affect the intended use of the protein.
Specifications
| Form: | Powder |
| Product Size: | 500 g |
| Reagent Type: | Chaotrope, Denaturant |
Contents & storage
- Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.
Note:
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