Human Episomal iPSC Line

Description

The Gibco Human Episomal iPSC Line was derived from CD34+ cord blood using a three-plasmid, seven-factor (SOKMNLT; SOX2, OCT4 (POU5F1), KLF4, MYC, NANOG, LIN28, and SV40L T antigen) EBNA-based episomal system. This iPSC line is considered to be zero foot-print as there was no integration into the genome from the reprogramming event.It has been shown to be free of all reprogramming genes and will provide researchers with a control line to compare and study a multitude of aspects of stem cell research.

The Gibco Human Episomal iPSC Line has a normal karyotype and endogenous expression of pluripotent markers like Oct4, Sox2, and Nanog (as shown by RT-PCR) and Oct4, SSEA4, TRA-1-60 and TRA-1-81 (as shown by ICC). Global gene expression analyses demonstrated that this episomal hiPSC line is molecularly indistinguishable from human embryonic stem cell lines (2). In directed differentiation and teratoma analyses, these hiPSCs retained their differentiation potential for the ectodermal, endodermal, and mesodermal lineages (1). In addition, vascular, hematopoietic, neural, and cardiac lineages were derived with robust efficiencies (1).

References
1. Burridge PW, Thompson S, Millrod MA, et al. 2011. A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One 6(4): e18293.
2. Quintanilla RH Jr, Asprer JST, Vaz C, et al. 2014. CD44 Is a Negative Cell Surface Marker for Pluripotent Stem Cell Identification during Human Fibroblast Reprogramming. PLoS One 9(1): e85419.

Specifications

Cell Type:	Stem Cells (iPS - Induced Pluripotent Stem)
Classification:	Animal Origin-Free
Culture Type:	Feeder Cell-Free
Media Recommendation:	Essential 8
Number of Cells:	1 x 10⁶
Product Size:	1 x 10⁶ cells⁄vial
Quantity:	1 vial(s)
Species:	Human

Contents & storage

1 vial cells, store in liquid nitrogen (vapor phase).

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