Pierce Premium Grade TCEP-HCl

Description

Thermo Scientific Pierce Premium Grade TCEP-HCl is our highest quality formulation of this disulfide reducing agent, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade TCEP-HCl:

• TCEP—a thiol-free, odorless yet potent reducing agent for cleaving disulfide bonds
• High quality—identity and purity confirmed by several tests, including quantitative NMR
• Product integrity—enhanced level of testing and characterization compared to standard grade
• Lot retention—ample supply of past lots retained to ensure future process testing
• Change management—Change Control Notification (CCN) service
• Consistent manufacture—batch-specific manufacturing documentation review

Compared to the standard grade product, Premium Grade TCEP-HCl provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable. TCEP selectively and completely reduces even the most stable water-soluble alkyl disulfides over a wide pH range.

Advantages of TCEP-HCl over other reducing agents:
• Odorless—Unlike DTT or BME, TCEP is odor-free, so reductions can be carried out conveniently on the bench top.
• Stable in air—The inherent stability of the TCEP moiety eliminates the need for any special precautions to avoid oxidation when handling, using or storing TCEP.
• Efficient—For most applications, 5 to 50 mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within a few minutes at room temperature.
• Compatible—With TCEP, removal of the reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, maleimide conjugations).

Specifications

Product Line: Pierce™
Product Size: 1 g

Contents & storage

Upon receipt store product at room temperature in sealed container to prevent oxidation. Product is shipped at ambient temperature.

Note:
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